Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials.
Identifieur interne : 000514 ( Main/Exploration ); précédent : 000513; suivant : 000515Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials.
Auteurs : Noelia Ayelen Boccardo [Argentine] ; María Eugenia Segretin [Argentine] ; Ingrid Hernandez [Cuba] ; Federico Gabriel Mirkin [Argentine] ; Osmani Chac N [Cuba] ; Yunior Lopez [Cuba] ; Orlando Borrás-Hidalgo [Cuba, République populaire de Chine] ; Fernando Félix Bravo-Almonacid [Argentine]Source :
- Scientific reports [ 2045-2322 ] ; 2019.
Descripteurs français
- KwdFr :
- Dosage biologique (MeSH), Environnement contrôlé (MeSH), Expression des gènes (MeSH), Glucan 1,3-beta-glucosidase (génétique), Phénotype (MeSH), Résistance à la maladie (génétique), Serine endopeptidases (génétique), Tabac (génétique), Tabac (immunologie), Tabac (microbiologie), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Glucan 1,3-beta-glucosidase, Résistance à la maladie, Serine endopeptidases, Tabac.
- immunologie : Tabac.
- microbiologie : Tabac.
- Dosage biologique, Environnement contrôlé, Expression des gènes, Phénotype, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Biological Assay (MeSH), Disease Resistance (genetics), Environment, Controlled (MeSH), Gene Expression (MeSH), Glucan 1,3-beta-Glucosidase (genetics), Phenotype (MeSH), Plants, Genetically Modified (MeSH), Serine Endopeptidases (genetics), Tobacco (genetics), Tobacco (immunology), Tobacco (microbiology).
- MESH :
- chemical , genetics : Glucan 1,3-beta-Glucosidase, Serine Endopeptidases.
- genetics : Disease Resistance, Tobacco.
- immunology : Tobacco.
- microbiology : Tobacco.
- Biological Assay, Environment, Controlled, Gene Expression, Phenotype, Plants, Genetically Modified.
Abstract
Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani, Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae. Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R. solani in greenhouse conditions and, furthermore, they are protected against P.hyoscyami f.sp. tabacina and P. nicotianae in field conditions under high inoculum pressure. Our results suggest that plastid co- expression of PR proteins AP24 and β-1,3-glucanase resulted in enhanced resistance against filamentous pathogens.
DOI: 10.1038/s41598-019-39568-6
PubMed: 30808937
PubMed Central: PMC6391382
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires</wicri:regionArea><wicri:noRegion>Ciudad Autónoma de Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Segretin, Maria Eugenia" sort="Segretin, Maria Eugenia" uniqKey="Segretin M" first="María Eugenia" last="Segretin">María Eugenia Segretin</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina. segretin@dna.uba.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires</wicri:regionArea><wicri:noRegion>Ciudad Autónoma de Buenos Aires</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (C1428EGA), Ciudad Autónoma de Buenos Aires, Argentina. segretin@dna.uba.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (C1428EGA), Ciudad Autónoma de Buenos Aires</wicri:regionArea><wicri:noRegion>Ciudad Autónoma de Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Hernandez, Ingrid" sort="Hernandez, Ingrid" uniqKey="Hernandez I" first="Ingrid" last="Hernandez">Ingrid Hernandez</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</nlm:affiliation><country xml:lang="fr">Cuba</country><wicri:regionArea>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana</wicri:regionArea><wicri:noRegion>La Habana</wicri:noRegion></affiliation></author><author><name sortKey="Mirkin, Federico Gabriel" sort="Mirkin, Federico Gabriel" uniqKey="Mirkin F" first="Federico Gabriel" last="Mirkin">Federico Gabriel Mirkin</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires</wicri:regionArea><wicri:noRegion>Ciudad Autónoma de Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Chac N, Osmani" sort="Chac N, Osmani" uniqKey="Chac N O" first="Osmani" last="Chac N">Osmani Chac N</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</nlm:affiliation><country xml:lang="fr">Cuba</country><wicri:regionArea>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana</wicri:regionArea><wicri:noRegion>La Habana</wicri:noRegion></affiliation></author><author><name sortKey="Lopez, Yunior" sort="Lopez, Yunior" uniqKey="Lopez Y" first="Yunior" last="Lopez">Yunior Lopez</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</nlm:affiliation><country xml:lang="fr">Cuba</country><wicri:regionArea>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana</wicri:regionArea><wicri:noRegion>La Habana</wicri:noRegion></affiliation></author><author><name sortKey="Borras Hidalgo, Orlando" sort="Borras Hidalgo, Orlando" uniqKey="Borras Hidalgo O" first="Orlando" last="Borrás-Hidalgo">Orlando Borrás-Hidalgo</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</nlm:affiliation><country xml:lang="fr">Cuba</country><wicri:regionArea>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana</wicri:regionArea><wicri:noRegion>La Habana</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Microbial Engineering, School of Biotechnology, Qi Lu University of Technology, Jinan, (250353), P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Microbial Engineering, School of Biotechnology, Qi Lu University of Technology, Jinan, (250353)</wicri:regionArea><wicri:noRegion>(250353)</wicri:noRegion></affiliation></author><author><name sortKey="Bravo Almonacid, Fernando Felix" sort="Bravo Almonacid, Fernando Felix" uniqKey="Bravo Almonacid F" first="Fernando Félix" last="Bravo-Almonacid">Fernando Félix Bravo-Almonacid</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires</wicri:regionArea><wicri:noRegion>Ciudad Autónoma de Buenos Aires</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, (B1876BXD), Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, (B1876BXD)</wicri:regionArea><wicri:noRegion>(B1876BXD)</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Assay (MeSH)</term><term>Disease Resistance (genetics)</term><term>Environment, Controlled (MeSH)</term><term>Gene Expression (MeSH)</term><term>Glucan 1,3-beta-Glucosidase (genetics)</term><term>Phenotype (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Serine Endopeptidases (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (immunology)</term><term>Tobacco (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Dosage biologique (MeSH)</term><term>Environnement contrôlé (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Glucan 1,3-beta-glucosidase (génétique)</term><term>Phénotype (MeSH)</term><term>Résistance à la maladie (génétique)</term><term>Serine endopeptidases (génétique)</term><term>Tabac (génétique)</term><term>Tabac (immunologie)</term><term>Tabac (microbiologie)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucan 1,3-beta-Glucosidase</term><term>Serine Endopeptidases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glucan 1,3-beta-glucosidase</term><term>Résistance à la maladie</term><term>Serine endopeptidases</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Assay</term><term>Environment, Controlled</term><term>Gene Expression</term><term>Phenotype</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dosage biologique</term><term>Environnement contrôlé</term><term>Expression des gènes</term><term>Phénotype</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani, Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae. Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R. solani in greenhouse conditions and, furthermore, they are protected against P.hyoscyami f.sp. tabacina and P. nicotianae in field conditions under high inoculum pressure. Our results suggest that plastid co- expression of PR proteins AP24 and β-1,3-glucanase resulted in enhanced resistance against filamentous pathogens.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30808937</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>02</Month><Day>26</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials.</ArticleTitle><Pagination><MedlinePgn>2791</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-39568-6</ELocationID><Abstract><AbstractText>Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani, Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae. Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R. solani in greenhouse conditions and, furthermore, they are protected against P.hyoscyami f.sp. tabacina and P. nicotianae in field conditions under high inoculum pressure. Our results suggest that plastid co- expression of PR proteins AP24 and β-1,3-glucanase resulted in enhanced resistance against filamentous pathogens.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boccardo</LastName><ForeName>Noelia Ayelen</ForeName><Initials>NA</Initials><AffiliationInfo><Affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Segretin</LastName><ForeName>María Eugenia</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina. segretin@dna.uba.ar.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (C1428EGA), Ciudad Autónoma de Buenos Aires, Argentina. segretin@dna.uba.ar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hernandez</LastName><ForeName>Ingrid</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mirkin</LastName><ForeName>Federico Gabriel</ForeName><Initials>FG</Initials><AffiliationInfo><Affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chacón</LastName><ForeName>Osmani</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lopez</LastName><ForeName>Yunior</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borrás-Hidalgo</LastName><ForeName>Orlando</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Centro de Ingeniería Genética y Biotecnología (CIGB), (10600), La Habana, Cuba.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Microbial Engineering, School of Biotechnology, Qi Lu University of Technology, Jinan, (250353), P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bravo-Almonacid</LastName><ForeName>Fernando Félix</ForeName><Initials>FF</Initials><AffiliationInfo><Affiliation>Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), (C1428ADN), Ciudad Autónoma de Buenos Aires, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, (B1876BXD), Argentina.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 3.2.1.58</RegistryNumber><NameOfSubstance UI="D043326">Glucan 1,3-beta-Glucosidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 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